Technology

Biocatalysis

Asymchem Technology Enzyme Lab

Cost-effective, Sustainable Approach to Transformation

Our experts can design, optimize and screen enzymes (biocatalysts) based on each project’s specific demands. Biotransformation (enzyme catalysis) is foundational to our manufacturing approach, guaranteed to meet GMP standards, and flexible enough to produce quantities from mg to ton manufacture. Synthetic route development results in a much more cost-effective and green process, taking advantage of the highly selective nature of enzymes, especially as a replacement of chiral precious metal catalysts.

On Site Catalyst Production Supports Full Chemical Process

Understanding the need to move away from proprietary enzymes offered by a single enzyme supplier, at Asymchem we optimize and produce catalysts on site that support the full chemical process. We immobilize each enzyme or modify its properties for maximum efficiency in a manufacturing environment. And because we manufacture and use these enzymes in a single location, we’re able to avoid loss of enzyme activity and enable the application of other advanced technologies.

Available Services

  • Enzyme Screening
  • Enzyme Development
  • Process Development & Optimization
  • Technology Transfer

Asymchem Enzymes Application and Opitmization Chart_APIs Intermediates Enzymes Chart

Enzyme Screening

Asymchem provides multiple enzyme screening kits that target a broad range of biotransformation, offering standalone enzyme screening against specific processes. When customers provide substrates which include analytical method development and process optimization, we will propose alternatives that add more value for less effort.

Enzyme Engineering

By producing enzyme solutions at the same site where the chemical process will be run, Asymchem avoids unnecessary steps making the enzyme suitable for shipment, while ultimately adding value. We can also seamlessly combine the biotransformation step with other technologies for the best overall process. Our enzyme engineering is chemist controlled, meaning our primary focus is to ensure effectiveness in a given step.

Our program features:

  • Recombinant expression
    • New enzyme development service for a specific substrate, including gene screening, recombinant expression, expression optimization, and evolution
  • Enzyme evolution technology
    • Rational design: The mutation sites are selected based on the simulated protein three-dimensional structure, for example as site-directed mutagenesis and saturation mutagenesis.
    • Irrational design: The mutation is random and diversity of variants is increased, for example error-prone PCR, DNA shuffling, and staggered extension process.
  • High through-put screening
    • This important part of enzyme evolution determines the efficiency and ability of the enzyme evolution. Asymchem has developed a series of high through-put screening methods for ketoreductase, transaminase, hydrolase, and other classes.
  • Enzyme immobilization
  • Downstream expertise
    • Expertise in adapting and optimizing customized DSP process based on enzyme — soluble and insoluble enzymes/proteins
  • Scalable DSP process from lab to production scale with maximum yield and activity
  • Experience in handling and scaling up instable and sensitive recombinant protein production

Immobilization Solutions

We offer a variety of enzyme immobilization solutions with the aim of achieving overall efficiency in integrated biocatalysis. Our immobilization platform is customized for enzymes with the purpose of suitability, scalability, and economies in the manufacturing process and offers the following advantages:

  • Quick and rational design
  • Generic and custom immobilization platforms
  • Single enzymes, multiple enzymes, and co-enzymes/cofactors
  •  Expertise in analysis method development
  • Operational stability testing of immobilized enzymes
  • PBR / CSTR mode of testing
  • Capability from gram to kilo scale

Enzyme Library

Asymchem’s enzyme library includes wild type enzymes in the public domain and enzymes engineered in house. All were produced by expression of recombinant genes. Stocks are continually maintained and replenished.

Enzyme Asymchem Enzyme Library Other Available Enzymes in Class Total
Ketoreductase 192 150 342
Transaminase 434 50 484
Hydrolase (lipase & esterase) 150 60 210
Amino acid dehydrogenase 86 30 116
Nitrilase 38 68 106
Ene reductases 50 23 73
Monooxygenase 149 96 245
Imine reductase 45 26 71
Ammonia Lyase 40 0 40
Proline hydroxylase 58 0 58
Threonine aldolase 35 0 35
Arylmalonic decarboxylase 25 0 25
Tyrosine phenol-lyase 21 0 21
Hydroxynitrile lyase 20 0 20
Amidohydrolase (Amidase & Acylase) 20 0 20
Other enzyme 81 104 185
Total 1444 607 2051

Other available enzymes: These samples in the Asymchem collection were obtained from outside sources, including other companies. Some are in the public domain, while others have IP encumbrances associated with their use.  Nevertheless, experiments with enzymes in this group sometimes lead to additions to the Asymchem Enzyme Library or help guide enzyme evolution work with Asymchem enzymes.

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